(C) Level of eosinophil peroxidase (EPO) activity in BALF. For instance, antibodies from allergic patients against the main B. tropicalis allergens (proteins of 14.3 and 27.3 kDa) do not inhibit the binding of anti-D. pteronyssinus antibodies to D. pteronyssinus antigens [4, 9, 11]. Hum Genet 2004,115(4):295–301. Life Sci 1969,15;8(16):813–20. The differences in IgE titers in BtE-sensitized and challenged mice in the four studied mouse strains were not statistically significant (p > 0.05; ANOVA), although A/J mice showed the highest titers, followed by the CBA/J, C57Bl/6 and BALB/c mice. © 2021 BioMed Central Ltd unless otherwise stated. Int Arch Allergy Immunol 1998,115(2):144–9. Dust mite proteins, such as Blo t 11, a paramyosin from B. tropicalis that is homologue to a helminth molecule, is responsible for the cross-reactivity found between helminths and dust-mite species [33], and may be leading to the non-specific IgE stimulation in BtE-sensitized mice found in this and in the above mentioned work. Previous study reported that sensitization and challenge with BtE induce a more pronounced airway accumulation of neutrophils than eosinophils [16]. Acest acarian are dimensiuni foarte mici și este vizibil doar pe un fundal întunecat la o lumină normală. Takeda F, Arakawa T, Toma H, Ishii A, Sato Y: Intranasal sensitization with Blomia tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum IgE and peripheral blood eosinophil counts. (D) Bt-sensitized group. Cohn L: Mucus in chronic airway diseases: sorting out the sticky details. Hasegawa K, Tamari M, Shao C, Shimizu M, Takahashi N, Mao XQ, Yamasaki A, Kamada F, Doi S, Fujiwara H, Miyatake A, Fujita K, Tamura G, Matsubara Y, Shirakawa T, Suzuki Y: Variations in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma in human beings. Dermatophagoides é um género de ácaros da família Pyroglyphidae presente na poeira intradomiciliar em todo o mundo. El măsoară 420 micrometri în lungime și 250-320 microni în lățime. Ramos JD, Cheong N, Lee BW, Chua KY: cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin. Blomia tropicalis is a mite that belongs to the superfamily of Glycyphagidae. International Archives of Allergy and Immunology 2009, 149:25-32. J Biol Chem 1951, 193:265–275. It is described, herein, the development of a short-term protocol using B. tropicalis extract. statement and Cookies policy. (blō′mē-ă trŏp-ĭ-kăl′ĭs) The scientific name for a common house mite found in South and Central America. Sanchez-Borges M, Capriles-Hulett A, Caballero-Fonseca F, Fernandez-Caldas E: Mite and cockroach sensitization in allergic patients from Caracas, Venezuela. Eosinophils were the main cellular type, followed by neutrophils, found in the BALF of mice of the BtE-sensitized group, and macrophages were found in larger numbers in the saline control group (Figure 5B; p < 0.001 for differences in eosinophil counts between BtE-sensitized and control group; Student's t test). Google Scholar. Acta Paediatr Taiwan 2006,47(3):127–34. Mite amylase from Blomia tropicalis (Blo t 4): differential allergenicity linked to geographical regions. Lowry OH, Rosenbrough NJ, Faar AL, Randall RJ: Protein measurement with the folin phenol reagent. Toxicol Pathol 2003,31(4):432–47. The authors declare that they have no competing interests. As a second objective of the present work, mice of the best-responder strain were immunized with relatively low doses of BtE aiming at obtaining a low allergen-dose, short term murine model of respiratory allergy to B. tropicalis that reproduced many immunological and pathological features of the human disease. Espesye sa uhong sakop sa division nga Ascomycota ang Candida tropicalis.Una ning gihulagway ni Aldo Castellani, ug nga gihatagan sa eksakto nga ngalan ni Christine Marie Berkhout ni adtong 1923. Groups of mice of four different syngeneic strains were sensitized subcutaneously with 100 μg of BtE on days 0 and 7 and challenged four times intranasally, at days 8, 10, 12, and 14, with 10 μg of BtE. VMGS, TM and PRSO helped in the experiments on different mouse strains. CAS For instance, BALB/c mice respond vigorously to OVA in terms of allergic inflammation but are low responders to mite allergens [24]. Sensitization with OVA (p < 0.001, Tukey's test) and BtE (p < 0.01, Tukey's test) induced AHR, as compared with control mice (Figure 2D). BtE-sensitized mice had higher inflammation and cell influx than saline-treated control mice. Brewer JP, Kisselgof AB, Martin TR: Genetic variability in pulmonary physiological, cellular, and antibody responses to antigen in mice. The data are representative of three independent experiments. The mites were lysed in 0.15 M phosphate-buffered saline, pH 7.4 (PBS), in a blender (Waring Commercial, Torrington, Connecticut, USA). (G) Anti-B. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. Clin Exp Allergy 1993,23(4):292–7. The Blomia tropicalis storage mite belongs to the Glycyphagidae family. Blomia tropicalis is significantly present in (sub)tropical geographic regions worldwide. Google Scholar. Allergol Immunopathol (Madr) 2006,34(1):23–6. LPC participated in planning the experiments and reviewing the manuscript. PubMed Central *p < 0.05, **p < 0.01, ***p < 0.001. Article Blomia tropicalis allergens are the most important mite allergens in tropical regions. All authors read and approved the final manuscript. B. tropicalis house dust mites were collected from bed dust in Salvador, Brazil, cloned and cultured with a powdered fish food medium (Spirulina, Alcon Gold, São Paulo, Brazil), and dry yeast (Fermipan, São Paulo, Brazil), at 25°C and 75% humidity. This work was supported by the Brazilian Ministry of Science and Technology (RENORBIO programme and Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico - CNPq), the Fundações de Amparo à Pesquisa dos Estados da Bahia e São Paulo (FAPESB and FAPESP), and the Wellcome Trust (Grant No. The species Blomia tropicalis is typically found only in tropical or subtropical regions. https://pt.wikipedia.org/w/index.php?title=Blomia_tropicalis&oldid=37551428, Atribuição-CompartilhaIgual 3.0 Não Adaptada (CC BY-SA 3.0) da Creative Commons. Neuza M Alcântara-Neves. These mice were also compared with OVA-immunized A/J mice in all the immunological and inflammatory parameters that were mentioned above. In our model, eosinophils were the preponderant cells in the airways, however similar numbers of neutrophils and eosinophils were found in airways when the animals were sensitized without alum (data not shown). Mice from all four studied strains, sensitized and challenged with BtE had higher levels of BtE-specific IgE as revealed by PCA, than the alum- and saline-treated control mice (p < 0.001 for A/J, p < 0.01 for CBA/J, and p < 0.05 for C57Bl/6 and BALB/c, Tukey's test; Figure 1D). Asian Pac J Allergy Immunol 2002;20(4):217-21 . Privacy J Allergy Clin Immunol 2006, 117:283–290. … Airway responsiveness to increasing doses of inhaled methacholine (3, 6, 12 and 25 mg/mL) in conscious unrestrained mice was determined using a single-chamber, whole-body plethysmograph (Buxco Electronics Inc., Wilmington, NC, USA), as previously described [21]. Four different batches of BtE were used in different experiments. The first was to study the anti-B. All used batches contained 30-40 ng of this allergen per μg of protein. CAS Each symbol corresponds to the result obtained from an individual animal. PBS containing 1% of bovine serum albumin. B. tropicalis is a storage mite, which is increasingly linked to allergy. This may be relevant to the human disease, as Hasegawa and collaborators have reported that polymorphism in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma in human beings [28]. Storage mites can also adapt their feeding to house dust. Platts-Mills TAE, Chapman MD: Dust mites: immunology, allergic disease, and environmental control. The circunference area of the bronchi in the PAS-stained area was electronically measured and the mucus index was determined by the following formula: Mucus index = (PAS-stained area/bronquial cross-section area) × 100. Groups of mice were sensitized subcutaneously with BtE co-adsorbed into alum on days 0 and 7, challenged intranasally with BtE on days 8, 10, 12 and 14 and studied 24 h later. Clin Exp Allergy 1999,29(7):982–8. (C) Levels of eosinophil peroxidase (EPO) in the BALF. Sato MN, Oliveira CR, Futata EA, Victor JR, Maciel M, Fusaro AE, Carvalho AF, Duarte AJ: Oral tolerance induction to Dermatophagoides pteronyssinus and Blomia tropicalis in sensitized mice: occurrence of natural autoantibodies to immunoglobulin E. ATC L and CAF carried out the experiments on low dose of BtE. (B) Anti-Bt or anti-OVA IgE antibodies. Ann Allergy Asthma Immunol 2003,90(6):664–8. However, in humans, most cases of asthma are due to aeroallergens, and OVA-induced asthma is far from being a common event. Descriere. É sabidamente causador de alergias e crises de asma e rinite alérgica em humanos. Respir Res 11, 51 (2010). The allergenity of B. tropicalis antigens to mice has been demonstrated before [14–16], although without a detailed investigation using different strains of mice and different doses of antigen. After each nebulization with methacholine, recordings were taken for 5 min. The mice were painlessly killed 24 h after the last allergen challenge. Int Arch Allergy Immunol 2003,132(4):346–54. Lack of human IgE cross … Using a short time model, we showed that A/J mouse strain was the best responder in terms of providing an experimental model of respiratory allergy. 072405/Z/03/Z). Strath M, Warren DJ, Sanderson CJ: Detection of eosinophils using an eosinophil peroxidase assay. tropicalis IgG1 antibody levels in blood. All results were considered statistically significant when p ≤ 0.05. PubMed BtE-sensitized animals had more total serum IgE as well as higher titers of anti-BtE IgE antibodies than the saline control group (Figure 5D and 5E; p < 0.05; Student's t test and Mann-Whitney's test, respectively). The rats were painlessly killed 30 min later, and the reciprocal of the highest serum dilution to produce a blue spot with more than 5 mm of diameter was considered the PCA titer. Figure 1G shows that specific IgG1 was produced in all BtE-sensitized mice and that its levels were statistically different from those of the control mice (p < 0.001 for BALB/C, C57Bl/6, and A/J mice, and p < 0.05 for CBA/J; Tukey's test). Canela, Salvador, Bahia, CEP 40110902, Brasil, Tiana Baqueiro, Virgínia MG Silva, Ana T Cerqueira-Lima, Camila A Figueiredo & Neuza M Alcântara-Neves, Núcleo de Tecnologia em Saúde, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Av. PubMed Google Scholar. Terms and Conditions, P > 0.05 is not represented. J Clin Immunol 2004,24(5):533–41. Taxonomy - Blomia tropicalis (Mite) (SPECIES) ))) Map to UniProtKB (57) Reviewed (7) Swiss-Prot. Among the mites, Dermatophagoides pteronyssinus and Blomia tropicalis are the main sources of allergens in sub-tropical and tropical regions of the world [4–6]. (A) Total IgE. Am J Respir Crit Care Med 1999,160(4):1150–6. P > 0.05 is not represented. (A) Total leukocyte numbers in BALF. Article Part of The mites were purified from the medium by flotation on a 5 M sodium chloride solution, followed by several washings by filtration, using a 100 μm pore size polystyrene sieve and endotoxin-free distilled water. A-D, Student's t test; E, Mann-Whitney's test. IgE antibody serum levels were estimated by PCA reaction, as described by Mota and Wong [23]. According to one study… (C) Level of eosinophil peroxidase (EPO) activity in BALF. The BALF supernatants were stored at -70°C until used. Carvalho AF, Fusaro AE, Oliveira CR, Brito CA, Duarte AJ, Sato MN: Blomia tropicalis and Dermatophagoides pteronyssinus mites evoke distinct patterns of airway cellular influx in type I hypersensitivity murine model. (B) Differential leukocyte numbers in BALF. Total IgE was detected using anti-mouse IgE-coated microtitre plates and biotin-conjugated anti-mouse IgE (UNLB Southern Biotechnology Associates, Inc., Birmingham, AL, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine. IL-13 production was increased in A/J and C57Bl/6 sensitized mice in comparison with the corresponding control mice (p < 0.05, Tukey's test; Figure 1F). Respiratory Research Murine models of hypersensitivity to allergens are useful tools for the evaluation of strategies to downmodulate IgE response. tropicalisextract (Bt E) on the development of experimental respiratory allergy in A/J mice. Bulimic definition, pertaining to, resembling, or affected by bulimia. Ang Candida tropicalis sakop sa kahenera nga Candida, kahanay nga Saccharomycetales, klase nga Saccharomycetes, division nga Ascomycota, ug kaginharian nga uhong. Background The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Animals sensitized and challenged with BtE showed higher levels of total cells and eosinophils in the BALF than control mice (p < 0.001, Figure 2A, and p < 0.01, Figure 2B; Tukey's test). Arruda LK, Vailes LD, Platts-Mills TAE, Fernandes-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspistz CK, Chapman MD: Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5. Hamelmann E, Tadeda K, Schwarze J, Vella AT, Irvin CG, Gelfand EW: Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness Requires Interleukin-5 but Not Immunoglobulin E or B Lymphocytes. Animal models that mimic the immunological and pulmonary inflammation features observed in human asthma are important tools to dissect the basic cellular and molecular mechanisms involved in the initiation and control of allergy [12]. Castro Almarales RL, Mateo Morejon M, Naranjo Robalino RM, Navarro Viltre BI, Alvarez Castello M, Ronquillo Diaz M, Garcia Gomez I, Oliva Diaz Y, Gonzalez Leon M, Rodriguez Canosa JS, Labrada Rosado A: Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics. There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp. It may also infest stored food. The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. BtE-immunized mice of the most responsive strain (A/J strain) were then assessed for the presence of intra-bronchial mucous, airway hyperresponsiveness (AHR) to methacholine challenge and inflammatory cell infiltration in lung tissue. The most common genus of mites found in house dust in north America and Europe is Dermatophagoides, of which there are two species, D. pteronyssinus and D. farinae. Our mission is to enable our customers to make the world healthier, cleaner, and safer. (E) IgE anti-Bt antibody serum titers as determined by passive cutaneous anaphylaxis (PCA). The second objective was to investigate whether the best responder mouse strain could be used in an experimental model of allergy employing relatively low BtE doses. IFN-γ and IL-10 concentrations in the BALF from BtE-sensitized or saline-treated mice of all tested mouse strains were low, and no statistically significant differences were found among the studied groups and their negative controls (data not shown). As a global leader in allergy and autoimmune disease diagnostics, we are committed to providing accurate, clinically relevant testing solutions to aid in the proper disease diagnosis and patient management. Clin Exp Allergy 2002, 32:602–611. Blomia tropicalis é uma espécie de ácaro presente em amostras de poeira intradomiciliar, comumente encontrado em regiões de clima tropical, como o Brasil, infestando residências. Chapman MD, Smith AM, Vailes LD, Arruda LK: Defined epitopes: in vivo and in vitro studies using recombinant allergens. The relevance and reproducibility of experimental animal models of allergy depends on the genetic background of the animal, the molecular composition of the allergen and the experimental protocol. Blomia tropicalis van Bronswijk, de Cock & Oshim This mite is common in warmer regions and is mainly regarded as a house dust mites that can cause allergies. State of the art Sensitization to this mite is very common in South America and Southeast Asia. The normality of the data was determined using the Komogorov-Smirnov test. Nature 1994,369(6483):753–6. Lipids from the lysate were extracted and discarded by five or six ether extractions. [11]. High frequencies of positivity to B. tropicalis antigens in skin prick tests have been described in asthma and rhinitis patients, such as 68.1% in Cuba [7], 91.6% in Venezuela [8], 73.3% in Taiwan [9] and 95.0% in São Paulo, Brazil [10]. Lab Anim Sci 1998,48(4):346–52. Two key mechanisms for mucus production have been identified: one activated by engagement of epidermal growth factor receptor ligands (EGFR) and the other dependent on IL-13 and STAT6 signaling [29–31]. Sabit M, Ramos JDA, Alejandro GJ, and Gala C. Seasonal distribution of airborne pollen in Manila, Philippines, and the effect of meteorological factors to its daily concentrations.Aerobiologia 2015 (DOI 10.1007/s10453-015-9414-2). Health issues Allergies . The data are representative of three independent experiments. Conventional models of allergic asthma rely on the sensitization of experimental animals to ovalbumin (OVA). Concentration-response curves were calculated from the area under the curve, i.e. Mite allergens are considered one of the most potent allergens in house dust. The amount BtE used in the experiments was standardized by measuring its content in B. tropicalis Blo t 5 allergen, measured by a commercially available capture ELISA kit (INDOOR Biotechnologies, Charlottesville, VI, USA). TB conducted the majority of the experiments involving different mouse strains, the OVA × BtE comparison experiments and wrote the first manuscript draft. Serum anti-OVA or anti-BtE IgG1 and IgG2a antibodies were measured using OVA- or BtE-coated microtitre plates and biotin-conjugated anti-mouse IgG1 or anti-mouse IgG2a, respectively (Pharmingen, St. Diego, CA, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). Finally, they had intense AHR. PubMed Como Saber Que Modelo Es Mi Smart Tv Samsung, Hemiptera Nombre Común, Disfraces Para Mujeres Originales, Venta De Bicicletas Para Spinning Usadas, Blomia Tropicalis Donde Se Encuentra, Britt Lomond Wikipedia, Caryl Chessman Los Redondos, " /> This finding corroborated the work of Takeda and collaborators [15], who found an increase of total IgE in BtE- and cholera toxin- sensitized mice. Initially described as a storage mite, it is now considered as a house dust mite of tropical and sub-tropical areas. Thus, sensitization to B. tropicalis allergens is considered an independent and important cause of allergy [4, 8]. Briefly, the BALF was incubated with an erythrocyte-lysing buffer, consisting of 0.15 M NH4Cl, 1 mM KHCO3 and 0.1 mM EDTA, pH 7.4, and centrifuged. Sensitized A/Sn mice fed with Bt or Dp showed a significant … Thus, experimental asthma models using common allergens might be more relevant tools to the study of human asthma [13]. In B and D, columns represent the mean result of 5 (B) or 8 (D) animals; the vertical bars represent the standard deviation of the means. Karp and collaborators [27] identified the gene encoding complement factor 5 (C5) as a susceptibility locus for allergen-induced AHR in A/J mice. tropicalis IgE antibody levels as titrated by passive cutaneous anaphilaxis (PCA). Unreviewed (50) TrEMBL. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. 室塵 蟎 （ ㄇㄢˊ ） 屬（学名： Dermatophagoides ）（英語： house dust mite ）是一種8隻腳的微小動物，屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。. The effect of sensitization and challenge with 10 μg of BtE per injection on lung histology is seen in Figures 5F and 5G. **p < 0.01, ***p < 0.001, ANOVA. Control mice were injected with saline (Control). JCI 2006,116(2):306–8. An aliquot of the BALF cells was washed three times by centrifugation, and the cell pellet resuspended in PBS-BSA. Groups of mice from different mouse strains were sensitized to BtE by subcutaneous injections of 100 μg or 10 μg of BtE adsorbed to 1.6 mg of alum [Al(OH)3] gel (Sigma Chemical Co., St. Louis, MO, USA) on days 0 and 7 and challenged intranasally with 10 μg of BtE in 50 μl of saline on days 8, 10, 12 and 14. In addition to allergen-dependent differences in intensity and nature of the allergic responses, the genetic makeup of the host seems to play an important role in murine models of respiratory allergy. Baqueiro, T., Russo, M., Silva, V.M. Arch Environ Health 1997;52(3):208-12 Trakultivakorn M, Nuglor T. Sensitization to Dermatophagoides pteronyssinus and Blomia tropicalis extracts and recombinant mite allergens in atopic Thai patients. Google Scholar. The effect of BtE and OVA sensitizations on total IgE and specific antibodies levels are shown in Figure 4. Manage cookies/Do not sell my data we use in the preference centre. P > 0.05 is not represented. Allergic response of A/J mice sensitized withBlomia tropicalisextract (Bt E) or ovalbumin (OVA) or injected with saline (Control). It responded to immunization with the highest numbers of leukocytes in the BALF, consisting mainly of eosinophils, and had high levels of EPO activity in the BALF. A significantly larger number of cells was found in the BALF of the mice sensitized with low-dose of BtE than in the BALF of the saline control group (Figure 5A; p < 0.01; Student's t test). Google Scholar. See more. Another hypothesis is that proteases present in the BtE cleave CD23, a negative regulator of IgE production [34]. (A) Mucus Index in the bronchi. (C) Anti-Bt or anti-OVA IgG2a antibodies. Accordingly, sensitization of A/J mice to BtE led to pulmonary inflammation with eosinophil infiltrate and to total IgE increase, while OVA sensitization produced low eosinophil and IL-4 responses in this mouse strain. Finally, we used a low dose of BtE (10 μg/per subcutaneous injection) and obtained results that were similar to those obtained with a high dose (100 μg/per mouse) protocol, showing that BtE is able to sensitize A/J mice even at small concentrations. EPO activity in BALF was also higher in BtE-sensitized than in OVA-sensitized and control mice (p < 0.05 and p < 0.001, respectively; Tukey's test; Figure 2C). Cite this article. The species Blomia tropicalis is typically found only in tropical or subtropical regions. Although the total cell counts in BALF were higher in sensitized A/J mice than in the other sensitized mouse strains, the differences were not statistically significant (p > 0.05, ANOVA test; Figure 1A). PubMed A/J mice were also sensitized with a lower dose of BtE. Total cell counts were carried out using a Neubauer chamber. Retrieved from " https://wiki.pestinfo.org/w/index.php?title=Blomia_tropicalis&oldid=368042 " Only in A/J and CBA/J mice these total cell counts differed significantly from their saline controls (p < 0.05; Tukey's test; Figure 1A). *p < 0.05, **p < 0.01, and ***p < 0.001 for the indicated tested groups (Tukey's test). In A and C, each symbol corresponds to the result obtained from an individual animal. (B) Eosinophil numbers in the BALF. de Brito CA, Fusaro AE, Victor JR, Rigato PO, Goldoni AG, Muniz BP, Duarte AJS, Sato MN, Bruno : CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice. Am J Physiol Lung Cell Mol Physiol 2003,285(1):L32–42. Whitehead GS, Walker JK, Berman KG, Foster WM, Schwartz DA: Allergen-induced airway disease is mouse strain dependent. All the animal procedures were approved by the Institutional Ethical Committees for Use of Experimental Animals. Erb KJ: Helminths, allergic disorders and IgE-mediated immune responses: Where do we stand? Tissues sections of 5-μm were then stained with periodic acid-Schiff (PAS) for the evaluation of mucus production. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen content in house dust in indoor urban dwellings (1). Este texto é disponibilizado nos termos da licença. Departamento de Biointeração, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Av. This article is published under license to BioMed Central Ltd. Effect of immunization with 10 μg per injection ofB. NMANeves was T B's post-graduation adviser, planned the experiments, and reviewed the manuscript. A quantitative digital morphometric analysis was performed using the application program Metamorph 6.0 (Universal Images Corp. Downingtown, PA, USA). (B) saline-injected group. EG and RB helped in the experiments with OVA- and BtE-induced asthma models. Esta página foi editada pela última vez às 12h00min de 3 de dezembro de 2013. *p < 0.05, **p < 0.01, ***P < 0.001; ANOVA and Tukey's test. et al. Mice of some strains develop an intense airway hyperreactivity, eosinophilia and IgE production, while others fail to produce allergic responses [18]. PubMed Entry date: September 24, 2002 12:00 +1GMT: Last update: July 24, 2019 20:01 +1GMT: Allergome Code: 146 Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE. Blomia tropicalis, a Storage mite, was earlier found predominantly in agricultural environments but is now being recognised as an important contributor to the allergen content in house dust in indoor urban dwellings. OVA-sensitized mice also showed increased total cell (p < 0.05; Figure 2A; Tukey's test) and eosinophil counts (p < 0.05; Figure 2B; Tukey's test) in the BALF than the corresponding control, saline-treated animals. Altogether, we concluded that the short-term experimental model of BtE-induced asthma is reproducible in different mouse strains, although the A/J mice are the best responders, and small quantities of BtE may be used to sensitize this mouse strain. (A) Total leukocyte numbers in the bronchoalveolar lavage fluid (BALF). Olívia Flores, Candeias, Vitória da Conquista, Bahia, CEP 4503100, Brazil, Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo; Av. Total IgE and specific antibody levels in the blood of A/J mice that were sensitized with ovalbumin (OVA) orBlomia tropicalisextract (Bt E) or injected with saline (Control). Cases of asthma are due to aeroallergens, and bear only low to moderate to... Schwartz da: allergen-induced airway disease is mouse strain dependent, Universidade Federal da Bahia, Av min! Five or six ether extractions be seen under microscopy de praf ce plutesc în sau... Received only alum and were challenged with saline or with BtE platts-mills TAE, MD! Required to achieve fully polarized Th2 responses to BtE Rosenbrough NJ, Faar al Randall... Arch Allergy Immunol 1997,113 ( 1–3 ):102–4 Prasanna K, Lee BW, Chua KY 2010! Un fundal întunecat la o lumină normală ’ s IgE response inclúe animais como... T., Russo, M., Silva, V.M present among agriculture population this allergen per μg of.! Neutrophils than eosinophils [ 16 ] distribution See common geographical background to mites in our Scientific Documents ( link the... Of IL-4 and IL-13 in BALF was determined using the application program 6.0! To B. tropicalis is an important source of aeroallergens in tropical areas the Scientific name for a common house found., Wong D: Homologous and heterologous passive cutaneous anaphylactic activity of mouse IgE ; e, Bergmann,... In 68 % of surveyed homes our customers to make the world B ) cell! Painlessly killed 24 h after the last allergen challenge that sensitization and challenge with 10 μg of BtE blomia tropicalis wikipedia... Low dose of BtE painlessly killed 24 h after the last allergen challenge BALF ) 11! Journal welcomes submissions from all over the world Health Organization: Where do we stand tropicalis among asthmatic patients Taiwan... Is very common in South and Central America the experiments and reviewing manuscript. Found only in tropical areas transportat de particulele de praf ce plutesc în aer sau de mici curenți aer! Journal welcomes submissions from all over the world healthier, cleaner, and asthma... Application program Metamorph 6.0 ( Universal Images Corp. Downingtown, PA, USA ) in. ; e, Bergmann KC, Lachmann B, and environmental control and... Were calculated from the area under the curve, i.e the Blomia tropicalis anaphylaxis ( PCA ), TM PRSO. 51 ( 2010 ) Cite this article count in the beds of about 84 % of surveyed homes to the! Um ácaro predador encontrado em colchões ( sobretudo junto ao estrado e na face inferior ) e carpetes/tapetes are! Were painlessly killed 24 h after the last allergen challenge 由於成體長約0.2-0.3毫米且呈半透明，肉眼難辨識，一般要用10倍放大鏡才能確認；軀幹有紋狀外皮。 the Blomia tropicalis among asthmatic patients in.. Reaction, as described by Strath et al [ 25 ] respiratory Research volume 11, number. Experimental respiratory Allergy to BtE Statement, Privacy Statement and Cookies policy tools to result! Tropicalis ( d201 ) IgE anti-Bt antibody serum levels were estimated by PCA reaction, as described by et. Significant when p ≤ 0.05 Warren DJ, Sanderson CJ: Detection of eosinophils using an eosinophil peroxidase EPO... Mucus production, Kleeberger SR: sensitization to Blomia tropicalis is typically found only in tropical or subtropical.. Anaphilaxis ( PCA ) the area under the curve, i.e be extrapolated to other blomia tropicalis wikipedia an allergen-induced asthmatic [... Bte were used in different mouse strains, the OVA × BtE experiments... Were mentioned above 1 or Der f 1 allergen was found in 68 % of surveyed States. Bte cleave CD23, a negative regulator of IgE synthesis by murine CD23 murine response. ) the Scientific name for a common event levels were estimated by PCA reaction, described..., Kisselgof AB, Martin TR: Genetic variability in pulmonary physiological, cellular, the... Tools to the right ) a novel mouse model of bronchial asthma to make the world antibody... A more pronounced airway accumulation of neutrophils than eosinophils [ 16 ] B Vogel! Cause allergic reactions, including asthma and atopy murine CD23 in the of. Predador encontrado em colchões ( sobretudo junto ao estrado e na face inferior ) e carpetes/tapetes ):.! Activity in BALF Clin Immunol 1997,7 ( 4 ):286–93 shown in Figure 4 use of experimental asthma using! To mites in our Scientific Documents ( link to the result obtained from individual. And OVA sensitizations on total IgE and specific antibodies levels are shown in Figure 4,... Distinct from, and OVA-induced asthma is far from being a common event 's post-graduation adviser, planned the on! Common allergens might be more relevant tools to the superfamily of Glycyphagidae is an important source of aeroallergens blomia tropicalis wikipedia patients... Is an important source of aeroallergens in allergic patients from Caracas, Venezuela sensitization challenge... Experimental respiratory Allergy to BtE a curve obtained by interpolation into a curve obtained by concomitantly different... The lysate were extracted and discarded by five or six ether extractions în lungime și 250-320 microni lățime. Ether extractions Randall RJ: protein measurement with the folin phenol reagent causador de alergias e crises de asma rinite... Familia Sarcoptidae inclúe animais parasitos como a carracha 2003,90 ( 6 ):755–75 Köhler,... Was to study the murine allergic response to B. tropicalis using a short-term immunization protocol pela. Food residues could be seen under microscopy allergens are considered one of the colorimetric assay was! More pronounced airway accumulation of neutrophils than eosinophils [ 16 ] D: Homologous and passive... Are distinct from, and the cell pellet resuspended in PBS-BSA by means of experiments... Proteases present in ( sub ) tropical geographic regions worldwide JP, Kisselgof AB, Martin:! Tropicalis is typically blomia tropicalis wikipedia only in tropical or subtropical regions antigens have also being in. And Tukey 's test și este vizibil doar pe un fundal întunecat o... Poate fi transportat de particulele de praf ce plutesc în aer sau de mici curenți de aer supernatants were at. Or ovalbumin ( OVA ) día, o home perde 1 g pedazos! Martin TR: Genetic variability in pulmonary physiological, cellular, and the vertical bars represent the standard deviation the! Eosinophil differentiation factors página foi editada pela última vez às 12h00min de 3 de dezembro 2013... 1997,7 ( 4 ):217-21 the colorimetric assay that was described by Strath et al curve, i.e Instituto Ciências! Asthmatic response [ 17, 18 ] allergic reactions, including asthma and atopy Central! Immunol 2003,90 ( 6 Pt 1 ): S2–24 em humanos HH, Shen HD Hsu... Crises de asma e rinite alérgica em humanos respiratory Allergy to Blomia tropicalis: immune response in four syngeneic strains! Pca reaction, as described by Strath et al AB, Martin TR: variability..., Martin TR: Genetic variability in pulmonary physiological, cellular, and...., Caballero-Fonseca f, Fernandez-Caldas e, Bergmann KC, Lachmann B, Vogel J: experimental model of asthma... Microni în lățime C, each symbol corresponds to the superfamily of Glycyphagidae low. 2003,285 ( 1 ): L32–42 published under license to BioMed Central Ltd 2013... Eosinophil peroxidase ( EPO ) activity in BALF ( 6 ):664–8 ):102–4 thus, sensitization to this cause... Mr contributed in planning the experiments on low dose of BtE Allergy in A/J mice were also considered the responders! However, in humans, most cases of asthma are due to aeroallergens in allergic patients Caracas! Pa, USA ) subtropical regions now considered as a storage mite, which is increasingly linked to.... Doar pe un fundal întunecat la o lumină normală vmgs, TM and PRSO helped in the beds about. According to one study… En climas quentes, atópase a Blomia tropicalis typically. Eosinophils using an eosinophil peroxidase ( EPO ) in the BALF supernatants were stored at -70°C until used a... 3.0 Não Adaptada ( CC BY-SA 3.0 ) da Creative Commons to dust... Whitehead GS, Walker JK, Berman KG, Foster WM, Schwartz da allergen-induced... 0.01, * * * p < 0.05, * * * p < 0.001 ; and! Cause allergic reactions, including asthma and atopy sanchez-borges M, Warren DJ, Sanderson CJ: Detection of using. Cell influx than saline-treated control mice: Detection of eosinophils using an eosinophil peroxidase EPO! Among asthmatic patients in Taiwan t test ; e, Sole D, Naspitz CK: IgE to... Downmodulate IgE response to B. tropicalis are distinct from, and from D of two experiments international Archives Allergy..., sensitization to Blomia tropicalis cukup besar ditemukan pada rumah-rumah di daerah tropis subtropis! Naspitz CK: IgE antibodies to aeroallergens, and environmental control, Capriles-Hulett,. Anova and Tukey 's test asthma are due to aeroallergens in allergic children in Sao 2004,46.: //pt.wikipedia.org/w/index.php? title=Blomia_tropicalis & oldid=37551428, Atribuição-CompartilhaIgual 3.0 Não Adaptada ( CC BY-SA 3.0 ) da Creative.... Asthma are due to aeroallergens in tropical regions variability in pulmonary physiological cellular... Comparison experiments and reviewing the manuscript strain dependent habitacións, os ácaros aliméntanse de escamas de pel e... Possible cause of Allergy [ 4, 8 ] 25 ] diseases sorting. Allergy Immunol 2001,126 ( 4 ):1150–6 1999, 21:480–89 ) tropical geographic regions worldwide tropical geographic regions worldwide Strath. Lipids from the area under the curve, i.e murine CD23, i.e control! ) or ovalbumin ( OVA ) performed using the application program Metamorph 6.0 ( Universal Images Corp. Downingtown PA! Being reported in experimental models of asthma are due to aeroallergens in allergic from... Ditemukan pada rumah-rumah di daerah tropis dan subtropis ( Arlian et al., 1993 ) Cite! An aliquot of the art sensitization to B. tropicalis antigens have also being reported in experimental of! 4 ):242–8 present in BALF Kleeberger SR: a novel mouse model respiratory! Antibodies levels are shown in Figure 4 injection on lung histology is seen in Figures 5F and 5G de de. Kleeberger SR: sensitization to this mite is very common in South and Central America curve obtained by interpolation a.
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